500 mg vial reconstituted with 5 mL bacteriostatic water
Purpose
Longevity
Route
SubQ
Vial
500 mg
Dose
100 mg
1 What It Does
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme central to cellular energy production and DNA repair. Levels decline with age; supplementation supports mitochondrial function, sirtuin activity, and metabolic health. Dosed in milligrams, not micrograms.
+Cellular energy (ATP)+DNA repair pathways+Sirtuin activation+Mitochondrial function+Metabolic support
2 Typical Protocol
Recommended dose
100 mg per dose
Frequency
2–3 days per week
Timing
AM
Duration
As needed
Inject SLOWLY
SubQ NAD+ can sting and cause a flushing/pressure sensation if pushed fast. Inject very slowly. A 100 mg dose is a full 1 mL syringe (100 units) — many split it or titrate up.
3 Reconstitution & Draw
Based on a 500 mg vial reconstituted with 5 mL BAC water. NAD+ is dosed in milligrams — note the scale.
500 mg vial · 5 mL water · 100 mg dose
BAC water added
Concentration
Draw for 100 mg
5 mL
100 mg/mL
100 units (full syringe)
10 mL
50 mg/mL
split over more water
Note
—
500 mg vial = five 100 mg doses
U-100 syringe: 100 units = 1 mL. At 5 mL water, 100 mg = a full 1 mL draw (100 units).
Comfort tip
If a full 100 mg dose stings, split it into two smaller injections or reconstitute with more water and dose more units over a larger volume, slowly.
4 Storage & Handling
Before mixing
Store lyophilized, cool & dark
After mixing
Refrigerate, use within 90 days
Reconstitute with
Bacteriostatic water
Handling
Inject very slowly — NAD+ stings if pushed fast
5 Where to Source
Trusted vendors with DPRO discount codes applied at checkout.
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Disclaimer
This cheat sheet is for educational and informational purposes only. It is not medical advice, not a prescription, and does not establish a doctor-patient relationship. Dosing ranges reflect commonly-cited figures and individual protocols vary. Many peptides are sold for research purposes only and may not be approved for human use. Consult a licensed physician before starting.